Trials of size: 100
Detection method: Absorbance (570 nm)
Species Reactivity: Mammalian
Applications: The kit can detect as little as 0.5 nmol per well or 10 µM of urea
• Urea Assay Buffer
• OxiRed probe in DMSO
• Enzyme blend (lyophilized)
• Converting enzyme
• Urea standard (100 mM)
Shipping conditions: Gel Pack
USE: For research use only! It is not for use in humans.
- Sensitive and precise. Use 5 µL samples. Linear detection range 0.08 mg/dL (13 µM) to 100 mg/dL (17 mM) urea in 96-well plate assay.
- Simple and high performance. The procedure involves the addition of a single working reagent and incubation for 20 min. It can be easily automated as a high throughput assay for thousands of samples per day.
- Improved stability and versatility of reagents. The optimized formulation has greatly improved the stability of reagents and signals. 96-well plate or cuvette assay.
- Low interference in biological samples. No previous treatments are needed. Assays can be performed directly on raw biological samples, ie, in the presence of lipids and proteins.
Urea assay kit is produced mainly in the liver and is secreted by the kidneys. Urea is the main end product of protein catabolism in animals. It is the main vehicle for the removal of toxic ammonia from the body. The determination of urea is very useful for the clinician to evaluate the renal function of patients. In general, elevated urea levels are associated with nephritis, renal ischemia, urinary tract obstruction, and certain extrarenal diseases, eg, congestive heart failure, liver disease, and diabetes. Reduced levels indicate acute liver failure or may be due to overly aggressive parenteral fluid therapy.
Simple, direct, and automation-ready procedures for measuring urea concentration or blood urea nitrogen BUN in biological samples are becoming popular in drug discovery and research. The BioAssay Systems Urea Assay Kit is designed to measure urea directly in biological samples without any pretreatment. The improved Jung method uses a chromogenic reagent that forms a specifically coloured complex with urea. The intensity of the colour, measured at 520nm, is directly proportional to the concentration of urea in the sample. The optimized formulation substantially reduces the interference of substances in raw samples.