Enzyme-Linked Immunosorbent Assay (ELISA)

Introduction The enzyme-linked immunosorbent assay (ELISA) is a powerful method for detecting and quantifying a specific protein in a complex mixture. Originally described by Engvall and Perlmann (1971), the method allows the analysis of protein samples immobilized in microplate wells using specific antibodies. ELISAs are generally performed in 96- or 384-well polystyrene plates, which passively …